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Release of Platelet Factor 3 Activity was Determined in Platelet Concentrate Units Collected by Apheresis by a New Procedure

Abstract by L. Cook, D. Bonderman, R. Speck, R. Larison and J Griep, University of Georgia Medical College Blood Bank, Augusta, GA. Nov., 15, 1996.

Propyl Gallate is a new, patented chemical activator which releases platelet factor 3 (PF3) and activates the factors in the intrinsic plasma coagulation pathway. This reagent was used to measure the release of platelet factor 3 in platelet concentrate units by an activated plasma clotting time (APCT) procedure (normal 23-40 seconds). The platelet concentrates were collected by apheresis, serially diluted with platelet depleted plasma (PDP) and assayed for APCT. The maximum dilution was determined which would support normal clotting. Platelet counts were also performed on each unit.

There was no correlation between the release of PF3 activity of the platelet concentrate unit and the platelet count nor the age of the unit. This initial study shows the variability of the release of PF3 activity in the platelet units collected by apheresis. The release of PF3 activity of the apheresis units may be an important criterion for selecting units to be used in platelet therapy.