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Activated Partial Thromboplastin Time

Sensitivity and Solubility, a New APTT

ACS’s Spectra™ APTT reagent offers true solubility, sensitivity to factors II, V, VIIIc, IX, X, XI, XII, Fletcher and Fitzgerald, an easy way to monitor low molecular weight heparin and unfractionated heparin, and an unprecedented ability to detect the lupus anticoagulant.

 

ACS’s Spectra™ APTT reagent is a special breakthrough in APTT reagents. Remarkably stable, truly soluble and very sensitive to six of the most clinically significant aspects of intrinsic pathway testing, Spectra™ offers the laboratorian incredible advantages over standard ellagic acid APTT reagents. Spectra™ APTT is more sensitive to Fletcher factor, Fitzgerald factor, factors V, VIIIc, IX, X, XII, unfractionated and low molecular weight heparin and the lupus anticoagulant and is equally sensitive to factor II and XI.

In addition to these outstanding performance characteristics such as those found in table 1, ACS’s Spectra™ APTT reagent has the added bonus of precise control on phospholipid content. This added precision keeps lot to lot variation to a minimum. ACS’s APTT reagent offers more than just extra sensitivity.

Sample

Spectra™ APCT

APTT (ACTIN™†)

Spectra™ APTT

Normal Platelet Rich Plasma

30.8 sec

27.5 sec

25.4 sec

Factor II Deficient

90.8 sec

56.3 sec

58.6 sec

Factor V Deficient

89.6 sec

62.6 sec

70.5 sec

Factor VII Deficient

36.1 sec

37.8 sec

35.1 sec

Factor VIIIc Deficient

73.1 sec

53.3 sec

62.7 sec

Factor IX Deficient

84.7 sec

52.4 sec

67.3 sec

Factor X Deficient

74.8 sec

47.6 sec

64.9 sec

Factor XI Deficient

69.2 sec

86.1 sec

71.4 sec

Factor XII Deficient

98.7 sec

62.4 sec

72.6 sec

Fletcher Deficient

51.9 sec

35.2 sec

50.4 sec

† Actin is a trademark of Dade Behring

Table 1. In this comparison of the sensitivity of Spectra™ APCT, Dade Behring's APTT (Actin†) and ACS's APTT (Spectra™) to different factor deficiencies, Spectra™ APCT shows sensitivity to V, VIIIc, X, XI and Fletcher Factors and shows much greater sensitivity to II, IX and XII than either APTT reagents.

 

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